本研究系统性地探索了通过结合神经元前体转录因子与形态发生素，从诱导性多能干细胞（iPSCs）中生成多样化人类神经元亚型的方法。研究人员设计了一套高通量筛选策略，利用单细胞RNA测序技术分析了近70万个细胞，覆盖了480种不同的形态发生素组合，从而识别出与人体内神经元相似的多种诱导性神经元（iNs）亚型，这些亚型在形态、功能和转录组上都表现出独特性。文章还深入揭示了形态发生素如何通过激活特定的基因调控网络来引导神经元分化，并通过基因敲除和过表达实验验证了这些调控网络的必要性和充分性。此外，研究比较了在诱导转录因子之前或之后进行模式化的不同方案，发现前者能产生更接近原代人类神经元的均匀细胞群。最后，此研究提出了一种普适性的策略，可用于编程其他多样化的细胞亚型，为理解人类生物学、疾病机制和未来细胞治疗提供了宝贵的数据集和方法论。References: Hsiu-Chuan Lin et al. Human neuron subtype programming via single-cell transcriptome-coupled patterning screens.Science389,eadn6121(2025).DOI:10.1126/science.adn6121
No persons identified in this episode.
This episode hasn't been transcribed yet
Help us prioritize this episode for transcription by upvoting it.
Popular episodes get transcribed faster
Other recent transcribed episodes
Transcribed and ready to explore now
Trump $82 Million Bond Spree, Brazil Tariffs 'Too High,' More
16 Nov 2025
Bloomberg News Now
Ex-Fed Gov Resigned After Rules Violations, Trump Buys $82 Mil of Bonds, More
16 Nov 2025
Bloomberg News Now
THIS TRUMP INTERVIEW WAS INSANE!
16 Nov 2025
HasanAbi
Epstein Emails and Trump's Alleged Involvement
15 Nov 2025
Conspiracy Theories Exploring The Unseen
New Epstein Emails Directly Implicate Trump - H3 Show #211
15 Nov 2025
H3 Podcast
Trump Humiliates Himself on FOX as They Call Him Out
15 Nov 2025
IHIP News