Dr. Layne Norton

And they infused essential amino acids for six hours and looked at skeletal muscle protein synthesis. And they found it went up and then came back down by two hours and then never went back up, right? Good experiment. Yeah, very interesting. So- We looked at a bunch of different things.

And they infused essential amino acids for six hours and looked at skeletal muscle protein synthesis. And they found it went up and then came back down by two hours and then never went back up, right? Good experiment. Yeah, very interesting. So- We looked at a bunch of different things.

The only thing we found that perhaps explained it a little bit, and I'm sure there's other labs that would argue with me on this. And again, this is in rat skeletal muscle, which, by the way, is a good model for human protein metabolism, but still. We looked at intracellular ATP levels and actually found that they were declining kind of in concert with the decline in muscle protein synthesis.

The only thing we found that perhaps explained it a little bit, and I'm sure there's other labs that would argue with me on this. And again, this is in rat skeletal muscle, which, by the way, is a good model for human protein metabolism, but still. We looked at intracellular ATP levels and actually found that they were declining kind of in concert with the decline in muscle protein synthesis.

The only thing we found that perhaps explained it a little bit, and I'm sure there's other labs that would argue with me on this. And again, this is in rat skeletal muscle, which, by the way, is a good model for human protein metabolism, but still. We looked at intracellular ATP levels and actually found that they were declining kind of in concert with the decline in muscle protein synthesis.

And muscle protein synthesis is an ATP-dependent process. But the process of protein turnover is energetically expensive. It's one of the reasons that protein has a higher thermic effect of food. And so our hypothesis was perhaps – By the effect of protein stimulating protein synthesis to start this machinery is energetically expensive enough that eventually you kind of run out of steam.

And muscle protein synthesis is an ATP-dependent process. But the process of protein turnover is energetically expensive. It's one of the reasons that protein has a higher thermic effect of food. And so our hypothesis was perhaps – By the effect of protein stimulating protein synthesis to start this machinery is energetically expensive enough that eventually you kind of run out of steam.

And muscle protein synthesis is an ATP-dependent process. But the process of protein turnover is energetically expensive. It's one of the reasons that protein has a higher thermic effect of food. And so our hypothesis was perhaps – By the effect of protein stimulating protein synthesis to start this machinery is energetically expensive enough that eventually you kind of run out of steam.

And so you have the signal there. But it just kind of ends, right? Now, there have been other experiments like Jordan Tromelin just published a paper a few months ago that got a bunch of feedback. It was 100 grams of protein after a resistance training exercise and saw that it was basically – like a lot more of it was used than we thought would be used.

And so you have the signal there. But it just kind of ends, right? Now, there have been other experiments like Jordan Tromelin just published a paper a few months ago that got a bunch of feedback. It was 100 grams of protein after a resistance training exercise and saw that it was basically – like a lot more of it was used than we thought would be used.

And so you have the signal there. But it just kind of ends, right? Now, there have been other experiments like Jordan Tromelin just published a paper a few months ago that got a bunch of feedback. It was 100 grams of protein after a resistance training exercise and saw that it was basically – like a lot more of it was used than we thought would be used.

Yeah.

Yeah.

Yeah.

So what I tell people is I don't make big shifts in my opinions based on single studies.

So what I tell people is I don't make big shifts in my opinions based on single studies.

So what I tell people is I don't make big shifts in my opinions based on single studies.

It shimmies me a little bit, right? And even before that study came out, what I had said is I think protein distribution matters, but I think it matters much, much less than total protein intake per day. Because all we need to do is look at some of these resistance training studies with intermittent fasting, right? where people are eating all their protein in an eight-hour window.

It shimmies me a little bit, right? And even before that study came out, what I had said is I think protein distribution matters, but I think it matters much, much less than total protein intake per day. Because all we need to do is look at some of these resistance training studies with intermittent fasting, right? where people are eating all their protein in an eight-hour window.

It shimmies me a little bit, right? And even before that study came out, what I had said is I think protein distribution matters, but I think it matters much, much less than total protein intake per day. Because all we need to do is look at some of these resistance training studies with intermittent fasting, right? where people are eating all their protein in an eight-hour window.