这篇资源文章介绍了 Perturb-Multimodal (Perturb-Multi)，一个整合了成像和测序的创新平台，用于在完整哺乳动物组织中进行池化基因筛选。该方法通过 RCA-MERFISH 捕获原位基因型和多重 RNA-蛋白质表型，并通过 固定细胞Perturb-seq 实现体内 CRISPR 筛选并保留完整的转录组。文章详细阐述了这些技术创新，并以小鼠肝脏为例，展示了其在探索肝功能遗传调控、细胞分区、应激反应和脂肪变性方面的应用。研究人员利用该平台生成了肝脏的多模式转录和形态组织图谱，并识别了在不同生理条件下影响细胞状态的关键基因调节因子，揭示了基因型-表型关系的新见解。References: Saunders, Reuben A. et al. Perturb-Multimodal: A platform for pooled genetic screens with imaging and sequencing in intact mammalian tissue.
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