这篇研究整合了三维成像与核分割技术，以高分辨率绘制组织微环境图谱。作者们使用人类胰腺组织作为模型，开发了一种新颖的工作流程，通过对连续切片进行三维重建和核形态分析，克服了传统二维组织学分析的局限性。这项技术能够识别细胞类型、量化核特征，并揭示细胞在三维空间中的复杂相互作用，例如白细胞在胰腺导管和血管附近的分布模式。研究结果强调了三维分析对于全面理解疾病进展中组织结构和细胞异质性的重要性，并为未来的数字病理学研究提供了强大的框架。References: Forjaz A, Kramer D, Shen Y, et al. Integration of nuclear morphology and 3D imaging to profile cellular neighborhoods[J]. biorxiv, 2025: 2025.03. 31.646356.
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