这篇文章对循环肿瘤细胞(CTCs)进行了全面的综述,CTCs是转移性癌症扩散的关键介质,可通过液体活检进行检测。文章讨论了CTCs的分子生物学特性及其临床应用潜力,重点介绍了用于CTCs富集和单细胞分析的技术进步,例如使用大血容量(如白细胞分离术)结合先进的微流控设备。尽管CTCs在某些临床应用中已被循环肿瘤DNA(ctDNA)超越,但新的技术和生物学见解正在重新确立CTCs在肿瘤异质性评估、免疫疗法指导以及早期癌症诊断中的重要性,有望提供与ctDNA互补的诊断作用。References: Dai C S, Mishra A, Edd J, et al. Circulating tumor cells: Blood-based detection, molecular biology, and clinical applications[J]. Cancer Cell, 2025, 43(8): 1399-1422.
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