这篇文章描述了一项新技术，该技术利用一个机器人非线性光学系统进行迭代多光子显微镜和光微加工，从而实现了异质组织的原位组织学。该技术通过时空聚焦激光烧蚀，将加工效率提高了 100 倍，解决了对颅脑系统等大型、不均匀样本进行亚微米分辨率三维成像的挑战。该系统整合了先进的流体控制和智能软件，确保了数天自主操作期间的稳定性和精度，最终在完整的鼠类颅脑系统中展示了全光学连续切片双光子显微镜的潜力，以实现精确的解剖重建。References: Ji X, Huang S, Friedman B, et al. Spatiotemporal focusing enables all-optical in situ histology of heterogeneous tissue[J]. Nature Methods, 2025: 1-6.
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