E1: Basics - from DNA to Sanger sequencing

Iuliia is confused about RNA and Daniel doesn’t know the size of a DNA nucleotideChapters:00:00-Intro02:00-DNA08:27-Enzymes13:05-RNAs, tRNAs16:18-mRNAs, µRNAs, lncRNAs, circRNAs21:35-Wobble23:30-PCR33:20-Sanger sequencing I36:30-Gel electrophoresis40:00-Sanger sequencing II53:10-Teaser next Episode, OutroTopics:DNA, RNA, Protein, PCR, Gel Electrophoresis, Sanger Sequencing- The Royal Institution on the DNA Discovery: https://www.youtube.com/watch?v=_w2453iom9s- The discovery of DNA on science history: https://sciencehistory.org/education/scientific-biographies/james-watson-francis-crick-maurice-wilkins-and-rosalind-franklin/- The New York Times on Franklins’ part: https://www.nytimes.com/2023/04/25/science/rosalind-franklin-dna.html- Wikipedias article on Proteins with history: https://en.wikipedia.org/wiki/Protein- Jrank on RNA: https://science.jrank.org/pages/5871/Ribonucleic-Acid-RNA-discovery-RNA.html- Microbe Notes showing the central dogma of molecular biology: https://microbenotes.com/central-dogma-replication-transcription-translation/- ThoughtCo on RNA and DNA: https://www.thoughtco.com/dna-versus-rna-608191- khanacademy explainers on PCR and Gel electrophoresis: https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/biotechnology/a/polymerase-chain-reaction-pcr- Amoeba Sisters on PCR: https://www.youtube.com/watch?v=a5jmdh9AnS4- Promega explaining Sanger sequencing: https://www.youtube.com/watch?v=dVRB4CaLizc- Microbe online’s writeup on Sanger sequencing: https://microbeonline.com/dna-sequencing-sanger-sequencing-method/- LibreTextsBiology on Proteins and Enzymes: https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Book%3A_General_Biology_(Boundless)/03%3A_Biological_Macromolecules/3.07%3A_Proteins_-_Types_and_Functions_of_Proteins- excedr on Taq Polymerase and PCR: https://www.excedr.com/resources/taq-polymerase-what-is-it-and-what-does-it-do/Since 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany.Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ).The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).P.S.: One DNA nucleotide is ~0.34 nanometers in „length“ - DRLicense NoticeThis podcast including all episodes, unless otherwise noted, is licensed under a ⁠Creative Commons Attribution 4.0 International License⁠.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.Attribution:Podcast Title: Explain PodcastCreators: Iuliia Novoselova, Daniel RickertWebsite: ⁠https://ngs-cn.de/explain-podcast/

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