Explain Podcast
Episodes
Arraysequencing
23 Dec 2025
Contributed by Lukas
For when matching and sticking is enough to get
Sequencing by Expansion (SBX)
18 Nov 2025
Contributed by Lukas
After 454 now the second try by Roche: Axelios.Correction: the 5B flow cell will arrive for the NovaseqX(+), not for the Nextseq2000Also:PacBio makes ...
NEWS: 23andMe
02 Oct 2025
Contributed by Lukas
This time something different.Sources:https://www.23andme.com/privacy/https://verbraucherschutzforum.berlin/2025-03-25/23andme-meldet-insolvenz-an-was...
SE5: NGS in Immunology
06 Aug 2025
Contributed by Lukas
The science of white blood cells, and much moreChapters:05:30 Immunology how?22:05 22q11.2 deletion syndrome34:30 Scientific collaboration40:00 Data l...
E15: Singular Genomics | ATAC-seq
28 May 2025
Contributed by Lukas
Sharing a flowcell, reinventedChapters:- 00:00 usegalaxy.org- 07:55 Singular Genomics start- 21:55 Max Reads- 29:40 DNA Structure / ATAC-seqSetup gala...
E14: Ultima Genomics + Element UltraQ
02 Apr 2025
Contributed by Lukas
Spinnin' bases on wafers to do mostly natural SBSChapters:- 00:00 Ultima ePCR with beads- 07:10 Spinning the wafer, nucleotides, no blocking- 14:3...
E13: Sequencing by Avidity | Element Biosciences + XLEAP
12 Mar 2025
Contributed by Lukas
Because sometimes a jellyfish prevents quenchingChapters:01:00 - XLeap update from Illumina08:00 - Avidites start, splitting the problems13:20 - In-de...
E12: PacBio ONSO | Sequencing by binding
28 Jan 2025
Contributed by Lukas
Because only one type of nucleotide is not complicated enoughChapters:01:35 Introducing old problems15:30 More Phred, more better34:40 QuickGen: genet...
SE4: Long vs Short reads
01 Aug 2024
Contributed by Lukas
Mixing up long and cheapChapters:00:00:00 Intro00:03:30 Florians history00:13:50 Q&A Ressources for Bioinformatics00:27:00 Long-read-tools.org00:3...
E11: MGI DNA Nanoball sequencing
02 Jul 2024
Contributed by Lukas
Rolling in circles to make DNA yarnsChapters:00:00 Bioinformatics Upper Crust Tools04:04 STAR05:00 MGI15:00 Daniels first contact20:00 Brief Market Ov...
E10: Genome Mapping
27 May 2024
Contributed by Lukas
Who needs all bases anyway?Chapters:00:00 Phasing08:50 Not Sequencing, really14:00 Bionano Saphyr Chip26:00 Nabsys - going electric29:30 Tools availab...
E9: Single Cell Sequencing
22 Mar 2024
Contributed by Lukas
Chapters:00:00 Gambling15:00 Bubbles30:00 Holes57:30 One more thing1:07:00 Finale1:08:00 Finale of season 1!Eric Chow explaining single cell sequencin...
SE3: PhiX / UMIs / QC
09 Feb 2024
Contributed by Lukas
Getting the most out of MachinesChapters:00:00 PhiX14:30 low complexity19:30 UMIs32:10 FastQC43:00 MultiQC56:40 PycoQCPhiX concentrations for loading ...
E8: Nanopore
16 Jan 2024
Contributed by Lukas
Pushing spaghetti through holesChapers:00:00 Intro11:15 Measuring Ions17:00 Quality23:00 Duplex24:30 MinION25:15 PromethION28:20 Methylation29:00 Dire...
E7: PacBio
21 Dec 2023
Contributed by Lukas
Zipping rounds in zeptolitersChapters:00:00 Intro 07:00 Laser09:00 Zeptoliters13:30 Polymerase speed19:00 Error rate22:00 SMRT Cell25:45 ccs30:00 Frag...
E6: Illumina Sequencing Part II
01 Dec 2023
Contributed by Lukas
Glued DNA bridges and rainbow clustersChapters:00:00-Flowcells03:40-DNA glued to glass09:00-Bridge amplification15:50-Cluster density and patterned Fl...
E5: Illumina Sequencing Part I
13 Nov 2023
Contributed by Lukas
Colour mixing in PHRED spaceChapters:00:00 Nucleotides level 209:00 Cycles12:00 Not-so-wobbly mutations15:30 Reducing colourspace22:30 Cutting connect...
SE2: How Sequencing is used
20 Oct 2023
Contributed by Lukas
Iuliia killed the stripey catChapters:00:00 Intro00:04:44 Stripes00:11:40 Sanger with colors00:17:20 CATCATCAT...00:33:20 Planes00:38:15 Genome assemb...
E4: Ion Torrent Sequencing
22 Sep 2023
Contributed by Lukas
H+ ions and where we can find themChapters:00:00 Intro04:26 pH08:04 DNA Polymerase reaction with H+12:30 Nucleotides16:30 Homopolymer problem, but dif...
E3: SOLiD Sequencing
22 Aug 2023
Contributed by Lukas
If ligation is more your thing- Special Operations explaining SOLiD Sequencing: https://www.youtube.com/watch?v=YLT-DUeaLms- Writeup by the Apollo Ins...
SE1: Kitchen Genomics - DIY DNA Isolation
04 Aug 2023
Contributed by Lukas
Iuliia and Daniel have fun with salt water and alcoholChapters:00:00:00 DIY DNA isolation 00:15:38 Directed evolution of EnzymesDNA isolation in your ...
E2: Pyrosequencing
14 Jul 2023
Contributed by Lukas
Daniel and Iuliia are confused about fireflies- Scientific american on how fireflies create light:https://www.scientificamerican.com/article/how-and-w...
E1: Basics - from DNA to Sanger sequencing
26 Jun 2023
Contributed by Lukas
Iuliia is confused about RNA and Daniel doesn’t know the size of a DNA nucleotideChapters:00:00-Intro02:00-DNA08:27-Enzymes13:05-RNAs, tRNAs16:18-mR...
Teaser
09 Jun 2023
Contributed by Lukas
The Explain Podcast hosts are bioinformatician Daniel and scientific coordinator Iuliia.We aim to EXPLAIN key next generation sequencing techniques an...