E7: PacBio

Zipping rounds in zeptolitersChapters:00:00 Intro 07:00 Laser09:00 Zeptoliters13:30 Polymerase speed19:00 Error rate22:00 SMRT Cell25:45 ccs30:00 Fragment size34:30 Megaruptor38:20 Data flow47:00 SVs53:00 MAS Seq- Starter: https://www.nature.com/articles/s41366-021-00824-3- Karobben on PacBio sequencing: https://karobben.github.io/2023/10/30/Bioinfor/PacBio/- bioinformticamente explaining the technology:https://bioinformaticamente.com/2020/12/05/pacbio-sequencing/- Basic Biochem explainer of SMRT sequencing:https://www.youtube.com/watch?v=H2gUmRf-K1c- Genomics Lab explaining it: https://www.youtube.com/watch?v=vRhUSjhygDU- PacBio explaining BAM Tags: https://pacbiofileformats.readthedocs.io/en/13.0/BAM.html- Megaruptor3 explainer (how we make the right sized fragments) :https://www.youtube.com/watch?v=zVhqnkQL8zo- How we select the right sized fragments: https://www.youtube.com/watch?v=A25C8xfBbq8Since 2018, West German Genome Center (https://wggc.de/)operates as the next generation sequencing facility and supportsresearch in genomics & transcriptomics by providing expertise andsequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/)- network of NGS core facilities in Germany. Listen to our podcast and we're happy to receive your feedback&questions via Email (podcast[dot]explain[at]gmail[dot]com ).The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).Our podcast is an outreach activity within the national genomicsfacility "West German Genome Center" funded by the DFG(https://www.dfg.de/) (No. 407493903).License NoticeThis podcast including all episodes, unless otherwise noted, is licensed under a ⁠⁠⁠Creative Commons Attribution 4.0 International License⁠⁠⁠.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.Attribution:Podcast Title: Explain PodcastCreators: Iuliia Novoselova, Daniel RickertWebsite: ⁠⁠⁠https://ngs-cn.de/explain-podcast/⁠

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