E4: Ion Torrent Sequencing

H+ ions and where we can find themChapters:00:00 Intro04:26 pH08:04 DNA Polymerase reaction with H+12:30 Nucleotides16:30 Homopolymer problem, but different19:30 PCR is mean to some bases24:30 AmpliSeq33:40 Today’s status38:58 cfDNA40:00 PHRED vs Qscore44:59 Chip scaling and prices51:00 Outro- bitesizedbio on the technology:https://bitesizebio.com/27399/all-in-the-chip-ion-torrent-sequencers/- Quick explainer from Basic Biochem: https://www.youtube.com/watch?v=vMUnN2Y48Us- Slideshow giving an overview:https://www.slideshare.net/USDBioinformatics/ion-torrent-sequencing- biobinge explaining Iontorrent sequencing:https://biobinge.pubpub.org/pub/its/release/1- rna-seqblog showing a comparison to Illumina:https://www.rna-seqblog.com/a-comparison-of-illumina-and-ion-torrent-sequencing-platforms-and-the-winner-is/- Explainer by Shomu’s Biology: https://www.youtube.com/watch?v=0Ch5189aaCUSince 2018, West German Genome Center (https://wggc.de/) operates as the next generation sequencing facility and supports research in genomics & transcriptomics by providing expertise and sequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/) - network of NGS core facilities in Germany.Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ).The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).License NoticeThis podcast including all episodes, unless otherwise noted, is licensed under a ⁠⁠⁠Creative Commons Attribution 4.0 International License⁠⁠⁠.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.Attribution:Podcast Title: Explain PodcastCreators: Iuliia Novoselova, Daniel RickertWebsite: ⁠⁠⁠https://ngs-cn.de/explain-podcast/⁠

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