In this episode, we dive into a milestone in C. elegans systems biology — the first application of SILAC-style metabolic proteome labelling in a whole animal. By feeding worms lysine auxotroph E. coli labelled with heavy lysine (Lys8), researchers enabled quantitative proteomics with precision typically reserved for cell culture.But it gets better — they made it RNAi compatible, allowing side-by-side comparisons of wild-type vs mutant proteomes in the same run.We discuss:How worms were labelled with heavy lysine using auxotrophic E. coliHow this enabled 94–97% incorporation of label in just one generationThe creation of RNAi-ready NJF01 bacteria for knockdown and labellingCase study: NHR-49 loss alters lipid metabolism proteins at scaleWhy this approach paves the way for whole-organism proteogenomics📖 Based on the research article:“Quantitative proteomics by amino acid labeling in C. elegans”Fredens, J., Engholm-Keller, K., Giessing, A., Pultz, D., Larsen, M.R., Højrup, P., Møller-Jensen, J., & Færgeman, N.J.Published in Nature Methods (2011)🔗 https://doi.org/10.1038/nmeth.1675🎧 Subscribe to the WOrM Podcast for more full-organism breakthroughs in metabolism, proteomics, and systems biology!🔗 www.veerenchauhan.com

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