Kevin McKernan

But on the backbone of that plasmid, you will have an antibiotic resistance gene. I think I had another one over there. It was like a plasmid map. If you just scroll one image over, like the, yeah, there you go. Bang. If you click on that, you'll see they have these little pieces on them. So the inserted gene would be the spike. That they put in there.

The spike is the sequence that they want to make RNA for the vaccine. That gets into a million cell will create a spike protein that your immune system theoretically will learn how to fight off COVID. Got it.

The spike is the sequence that they want to make RNA for the vaccine. That gets into a million cell will create a spike protein that your immune system theoretically will learn how to fight off COVID. Got it.

The spike is the sequence that they want to make RNA for the vaccine. That gets into a million cell will create a spike protein that your immune system theoretically will learn how to fight off COVID. Got it.

There's a huge leap between A and Z there. Okay. That we'll go into. Okay.

There's a huge leap between A and Z there. Okay. That we'll go into. Okay.

There's a huge leap between A and Z there. Okay. That we'll go into. Okay.

Yeah. So that inserted gene ends up being, they put in the spike sequence there. There's an origin of replication down there that teaches the E. coli cell to, hey, copy this circle over and over again if you ever see me. It basically recruits the polymerase to do that.

Yeah. So that inserted gene ends up being, they put in the spike sequence there. There's an origin of replication down there that teaches the E. coli cell to, hey, copy this circle over and over again if you ever see me. It basically recruits the polymerase to do that.

Yeah. So that inserted gene ends up being, they put in the spike sequence there. There's an origin of replication down there that teaches the E. coli cell to, hey, copy this circle over and over again if you ever see me. It basically recruits the polymerase to do that.

That antibiotic resistance gene, let's say that's kanamycin in the case of Pfizer's vaccine. That means if the coli is growing in kanamycin, the only cells that can survive are the ones that have soaked up the plasmid. So it's a selectable marker, which means the only coli that brew are ones that contain your DNA. Otherwise, when the cells divide, your plasma just gets lost.

That antibiotic resistance gene, let's say that's kanamycin in the case of Pfizer's vaccine. That means if the coli is growing in kanamycin, the only cells that can survive are the ones that have soaked up the plasmid. So it's a selectable marker, which means the only coli that brew are ones that contain your DNA. Otherwise, when the cells divide, your plasma just gets lost.

That antibiotic resistance gene, let's say that's kanamycin in the case of Pfizer's vaccine. That means if the coli is growing in kanamycin, the only cells that can survive are the ones that have soaked up the plasmid. So it's a selectable marker, which means the only coli that brew are ones that contain your DNA. Otherwise, when the cells divide, your plasma just gets lost.

So that selectable marker is really critical to make sure the plasmid sticks around when you've grown a coli. So once you have your gene in a system like this, all you need to do to get the new coli is you heat a coli to like 37 degrees and it gets porous and it soaks up the plasmids. And then it has the superpower of being resistant to kanamycin.

So that selectable marker is really critical to make sure the plasmid sticks around when you've grown a coli. So once you have your gene in a system like this, all you need to do to get the new coli is you heat a coli to like 37 degrees and it gets porous and it soaks up the plasmids. And then it has the superpower of being resistant to kanamycin.

So that selectable marker is really critical to make sure the plasmid sticks around when you've grown a coli. So once you have your gene in a system like this, all you need to do to get the new coli is you heat a coli to like 37 degrees and it gets porous and it soaks up the plasmids. And then it has the superpower of being resistant to kanamycin.

And so now only the cells that soaked up the DNA can survive in the growth. They start replicating and now they're replicating your hijacked spike sequence with this.

And so now only the cells that soaked up the DNA can survive in the growth. They start replicating and now they're replicating your hijacked spike sequence with this.

And so now only the cells that soaked up the DNA can survive in the growth. They start replicating and now they're replicating your hijacked spike sequence with this.

So the challenge here is, is that you now don't just have your spike sequence as part of the contaminant in your vaccine. You have all that other crap, the antibiotic resistance gene and all the origins of replication that you introduced as replicative machinery to manufacture your.