Kevin McKernan
speaker
709 appearances
2 recordings
2 series
first heard Nov 2024
last heard 5 Dec
Kevin McKernan’s voice in public audio — every appearance, attributed to the second.
Trend
recordings per month · last 12 monthsRecordings per month over the last 12 months — 1 in all, peaking in Dec 2025 with 1.
Appearances
So they have a spike sequence attached to a ketamycin resistance gene attached to what's known as a bacterial origin of replication that a coli will grab onto and replicate this DNA. Once that's in a bacterial cell, you just have to grow that stuff at 37 degrees overnight and you get millions of old amplification of the cells. They double every 20 or 30 minutes.
And so every time the cell doubles, you get about 200 copies of that piece of DNA with it. So fermenting this overnight and suddenly you get yourself millions and millions of copies of this DNA. that you now need to get out of a coli. So you lice it open with some soap and purify that DNA, hopefully away from all the other junk. And now you've got your plasmid purified, ready to turn into RNA.
That's kind of the manufacturing process that they use for making these Pfizer vaccines. Now, what is a plasmid? A plasmid is that circular piece of DNA that kind of carries the spike DNA that you want to have replicated in a coli. So it contains, the plasmid is circular, so it contains an antibiotic resistance gene and an origin of replication that ensures the coli cell will replicate it.
So if you glue your spike sequence onto that piece of DNA into a circle, put that in a coli, and give it an antibiotic, the only coli that will survive are the coli cells that have the plasmid that code for the resistance. Okay. Oh, there you go. Nice. Plasma maps. So you can see a bacterial DNA in there. The bacterial DNA in a coli is like six million letters long.
These plasmas are like 5,000 to 10,000 letters long.
But they're circular. That helps them replicate and through a process known as rolling circle amplification, but also keeps them from degrading as quickly because there's no ends of DNA. When they're tied in a Mobius strip like that, the enzymes don't know how to really destroy them. So plasmas tend to stick around for a very long period of time.
But on the backbone of that plasmid, you will have an antibiotic resistance gene. I think I had another one over there. It was like a plasmid map. If you just scroll one image over, like the, yeah, there you go. Bang. If you click on that, you'll see they have these little pieces on them. So the inserted gene would be the spike. That they put in there.
The spike is the sequence that they want to make RNA for the vaccine. That gets into a million cell will create a spike protein that your immune system theoretically will learn how to fight off COVID. Got it.
There's a huge leap between A and Z there. Okay. That we'll go into. Okay.
Yeah. So that inserted gene ends up being, they put in the spike sequence there. There's an origin of replication down there that teaches the E. coli cell to, hey, copy this circle over and over again if you ever see me. It basically recruits the polymerase to do that.
That antibiotic resistance gene, let's say that's kanamycin in the case of Pfizer's vaccine. That means if the coli is growing in kanamycin, the only cells that can survive are the ones that have soaked up the plasmid. So it's a selectable marker, which means the only coli that brew are ones that contain your DNA. Otherwise, when the cells divide, your plasma just gets lost.
So that selectable marker is really critical to make sure the plasmid sticks around when you've grown a coli. So once you have your gene in a system like this, all you need to do to get the new coli is you heat a coli to like 37 degrees and it gets porous and it soaks up the plasmids. And then it has the superpower of being resistant to kanamycin.
And so now only the cells that soaked up the DNA can survive in the growth. They start replicating and now they're replicating your hijacked spike sequence with this.
So the challenge here is, is that you now don't just have your spike sequence as part of the contaminant in your vaccine. You have all that other crap, the antibiotic resistance gene and all the origins of replication that you introduced as replicative machinery to manufacture your.
They know it.
They try. They fail. But that's the point where they kind of. Is this in all vaccines? No, these mRNA ones are very unique, right? We've never done vaccines like this before.
They're just rolling out RSV and I think flu ones now. Okay. There's really scary ones going on in Japan right now. They're trialing out a self-amplifying mRNA vaccine, which is horrifying. That is something that's likely going to spread out of control. So there's not many.
First one we had. Okay. Yes. So it was a complete unknown.
Moderna had a better protocol. In fact, what's really key about this whole story is if you read Moderna's patents, they speak to this problem. That one, it's very difficult to figure out how much of this is left behind with PCR alone. So Stefan Bansal has a patent that says don't use qPCR to measure what's left behind. But they also have some other patents in there on techniques to get rid of it.
And the reason it is so important to get rid of is they write in that patent showing that it's oncogenic. If you leave this DNA around, it will insert into your genome and can create cancer. So you have to get rid of it. And that's written straight into their own patent estate.
Showing 381–400 of 709 · page 20 of 36
← Previous
Next →