Kevin McKernan

But when I kept getting them, I just kept throwing them in the freezer being, I don't know what to do with these things. And then this cannabis experiment I mentioned earlier was like, okay – We have a problem. I need a pharmaceutical grade vaccine. Maybe I need pharmaceutical grade mRNA. And I'm like, these things are sitting in the freezer. Just spike one of those in. It's got a poly-A tail.

But when I kept getting them, I just kept throwing them in the freezer being, I don't know what to do with these things. And then this cannabis experiment I mentioned earlier was like, okay – We have a problem. I need a pharmaceutical grade vaccine. Maybe I need pharmaceutical grade mRNA. And I'm like, these things are sitting in the freezer. Just spike one of those in. It's got a poly-A tail.

That should tell us what's going on with the RNA-seq that we're doing. And it did, but out came the vector that they used to manufacture these things, and that shouldn't be in there.

That should tell us what's going on with the RNA-seq that we're doing. And it did, but out came the vector that they used to manufacture these things, and that shouldn't be in there.

That should tell us what's going on with the RNA-seq that we're doing. And it did, but out came the vector that they used to manufacture these things, and that shouldn't be in there.

So when they make these mRNAs, they have to have a DNA template to make it from. So all RNA is actually synthesized usually from some other piece of DNA. Okay. Think of it as like a mold if you're into injection molding. You need something to kind of print the RNA. The DNA, they have to make it in the form of DNA first. So they take that spike protein sequence and they first code it into DNA.

So when they make these mRNAs, they have to have a DNA template to make it from. So all RNA is actually synthesized usually from some other piece of DNA. Okay. Think of it as like a mold if you're into injection molding. You need something to kind of print the RNA. The DNA, they have to make it in the form of DNA first. So they take that spike protein sequence and they first code it into DNA.

So when they make these mRNAs, they have to have a DNA template to make it from. So all RNA is actually synthesized usually from some other piece of DNA. Okay. Think of it as like a mold if you're into injection molding. You need something to kind of print the RNA. The DNA, they have to make it in the form of DNA first. So they take that spike protein sequence and they first code it into DNA.

And then they use an enzyme known as T7 polymerase to copy the DNA and turn it into RNA. And when they're doing that process, in the case of these mRNA vaccines, they rip out one of the bases and put in this pseudouridine so that there's only a pseudouridine and there's no uracil. That's how they get these mRNAs made.

And then they use an enzyme known as T7 polymerase to copy the DNA and turn it into RNA. And when they're doing that process, in the case of these mRNA vaccines, they rip out one of the bases and put in this pseudouridine so that there's only a pseudouridine and there's no uracil. That's how they get these mRNAs made.

And then they use an enzyme known as T7 polymerase to copy the DNA and turn it into RNA. And when they're doing that process, in the case of these mRNA vaccines, they rip out one of the bases and put in this pseudouridine so that there's only a pseudouridine and there's no uracil. That's how they get these mRNAs made.

Now, that means they have to have a piece of DNA that codes for the spike sequence they want to make the turn into RNA, but they need something to propagate that DNA. DNA is not going to organize itself. So what they do is they put a couple other genes on that DNA that make it so a coli will replicate it. So a what? That a coli, a bacteria. So basically you can brew it.

Now, that means they have to have a piece of DNA that codes for the spike sequence they want to make the turn into RNA, but they need something to propagate that DNA. DNA is not going to organize itself. So what they do is they put a couple other genes on that DNA that make it so a coli will replicate it. So a what? That a coli, a bacteria. So basically you can brew it.

Now, that means they have to have a piece of DNA that codes for the spike sequence they want to make the turn into RNA, but they need something to propagate that DNA. DNA is not going to organize itself. So what they do is they put a couple other genes on that DNA that make it so a coli will replicate it. So a what? That a coli, a bacteria. So basically you can brew it.

And this is, I mean, we did this in the Human Genome Project, 20 million different clones. It's a very common biotech thing. What you do is you put an antibiotic resistance gene attached to the spike sequence. And that antibiotic resistance gene and a couple other pieces of sequence guarantee that it will grow inside of a coli.

And this is, I mean, we did this in the Human Genome Project, 20 million different clones. It's a very common biotech thing. What you do is you put an antibiotic resistance gene attached to the spike sequence. And that antibiotic resistance gene and a couple other pieces of sequence guarantee that it will grow inside of a coli.

And this is, I mean, we did this in the Human Genome Project, 20 million different clones. It's a very common biotech thing. What you do is you put an antibiotic resistance gene attached to the spike sequence. And that antibiotic resistance gene and a couple other pieces of sequence guarantee that it will grow inside of a coli.

So when you put that DNA into a coli, you can then brew up huge vats of a coli And now it's copying your plasmid as long as there is a piece, there's antibiotics in there that match the gene. So in this case, they were using, what do they have? I think they use ketamycin in this case. Ketamycin is an antibiotic.

So when you put that DNA into a coli, you can then brew up huge vats of a coli And now it's copying your plasmid as long as there is a piece, there's antibiotics in there that match the gene. So in this case, they were using, what do they have? I think they use ketamycin in this case. Ketamycin is an antibiotic.

So when you put that DNA into a coli, you can then brew up huge vats of a coli And now it's copying your plasmid as long as there is a piece, there's antibiotics in there that match the gene. So in this case, they were using, what do they have? I think they use ketamycin in this case. Ketamycin is an antibiotic.